Hotspot

ndhG-ndhI

SSC · 123871-124263 · 393 bp

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Pi 0.0103
MarkerSeek score 61.60
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
393
variable_sites
4
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 ACCACTCCTAGACCTCCTAA AAACCGGGTAAACCTCTTGA 50.0 45.0 56.1 56.7 514-518 bp 1.0 55.3
ndhG-ndhI_p2 ACCACTCCTAGACCTCCTAA CCGGGTAAACCTCTTGATTCA 50.0 47.6 56.1 57.7 511-515 bp 1.0 55.2
ndhG-ndhI_p3 ACCACTCCTAGACCTCCTAA ACCGGGTAAACCTCTTGATTC 50.0 47.6 56.1 57.7 512-516 bp 1.0 55.2
ndhG-ndhI_p4 ACCACTCCTAGACCTCCTAA AACCGGGTAAACCTCTTGAT 50.0 45.0 56.1 56.4 513-517 bp 1.0 53.5
ndhG-ndhI_p5 ACCACTCCTAGACCTCCTAA ACCGGGTAAACCTCTTGATT 50.0 45.0 56.1 56.4 512-516 bp 1.0 53.5