Hotspot

ccsA-ndhD

SSC · 120417-120630 · 214 bp

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Pi 0.028

MarkerSeek score 65.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 214
variable_sites: 6
pi: 0.028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCAATTGTGGCTTCTA	TCCGGATTTCGTTCTCTCAC	45.0	50.0	57.8	57.4	399-405 bp	1.0	74.5
ccsA-ndhD_p2	TTCCGCAATTGTGGCTTCTA	CCGGATTTCGTTCTCTCACT	45.0	50.0	57.8	57.3	398-404 bp	1.0	74.4
ccsA-ndhD_p3	AATTCCGCAATTGTGGCTTC	TCCGGATTTCGTTCTCTCAC	45.0	50.0	57.6	57.4	401-407 bp	1.0	73.0
ccsA-ndhD_p4	AATTCCGCAATTGTGGCTTC	CCGGATTTCGTTCTCTCACT	45.0	50.0	57.6	57.3	400-406 bp	1.0	73.0
ccsA-ndhD_p5	TCCGCAATTGTGGCTTCTAT	TCCGGATTTCGTTCTCTCAC	45.0	50.0	57.6	57.4	398-404 bp	1.0	72.7