Hotspot

petA-psbJ

LSC · 66484-67644 · 1161 bp

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Pi 0.0121

MarkerSeek score 52.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1161
variable_sites: 14
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGACGCCAAGTGGTTGATAT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1991-1997 bp	1.0	80.6
petA-psbJ_p2	GGACGCCAAGTGGTTGATAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1990-1996 bp	1.0	80.6
petA-psbJ_p3	ATAGGTCCTGTTTCCGGTCA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.1	58.0	2245-2251 bp	1.0	80.4
petA-psbJ_p4	AAGGGGCTTTAAATGTGGGG	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2398 bp	0.5	59.6
petA-psbJ_p5	AAGGGGCTTTAAATGTGGGG	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2397 bp	0.5	59.6