Hotspot

atpB-rbcL

LSC · 57938-58668 · 731 bp

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Pi 0.0041

MarkerSeek score 61.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 3
pi: 0.0041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	877 bp	1.0	71.8
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	876 bp	1.0	71.5
atpB-rbcL_p3	ATCCAGTACCGGACCAATGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	876 bp	1.0	71.1
atpB-rbcL_p4	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	875 bp	1.0	71.1
atpB-rbcL_p5	AAACCCCAGGACCAGAAGTA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	817 bp	1.0	71.1