Hotspot

psbM-trnD-GUC

LSC · 31401-32524 · 1124 bp

Back to Hylomecon

Pi 0.00445

MarkerSeek score 61.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1124
variable_sites: 5
pi: 0.00445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTGGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.8	58.5	1235-1279 bp	1.0	70.4
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTGGC	GTTCAATTGGTCAGAGCACC	50.0	50.0	58.8	57.0	1236-1280 bp	1.0	66.3
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTGGC	TCAATTGGTCAGAGCACCG	50.0	52.6	58.8	57.8	1234-1278 bp	1.0	64.4
psbM-trnD-GUC_p4	TAGAATGAACAGTGCAGTGGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.6	58.5	1236-1280 bp	1.0	63.9
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTGGC	AGTTCAATTGGTCAGAGCACC	50.0	47.6	58.8	58.5	1237-1281 bp	1.0	62.3