Hotspot

rps2-rpoC2

LSC · 17282-17493 · 212 bp

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Pi 0.0142

MarkerSeek score 61.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 3
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	TTGCAGAGATGTAAGGTGCC	CCATCAGAACAATCATTTCCAGG	50.0	43.5	57.9	57.7	362-363 bp	1.0	53.9
rps2-rpoC2_p2	TTGCAGAGATGTAAGGTGCC	TCCATGATCCATCAGAACAATCA	50.0	39.1	57.9	57.6	370-371 bp	1.0	52.9
rps2-rpoC2_p3	GCAGAGATGTAAGGTGCCAT	CCATCAGAACAATCATTTCCAGG	50.0	43.5	57.7	57.7	360-361 bp	1.0	52.1
rps2-rpoC2_p4	GCAGAGATGTAAGGTGCCAT	TCCATGATCCATCAGAACAATCA	50.0	39.1	57.7	57.6	368-369 bp	1.0	51.1
rps2-rpoC2_p5	TTGCAGAGATGTAAGGTGCC	TGCATCCAAAAGAATTTCCATGA	50.0	34.8	57.9	57.3	386-387 bp	1.0	50.0