Hotspot

rps16-trnQ-UUG

LSC · 5913-7789 · 1877 bp

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Pi 0.00906
MarkerSeek score 58.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1877
variable_sites
17
pi
0.00906
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1968-1983 bp 1.0 79.9
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1966-1981 bp 1.0 79.8
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1974-1989 bp 1.0 79.7
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1975-1990 bp 1.0 79.7
rps16-trnQ-UUG_p5 GTCGAGCTAAGAGCACCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 2021-2036 bp 1.0 79.4