Hotspot

ycf2

LSC · 86705-93373 · 6669 bp

Back to Hygrophila

Pi 0.0249

MarkerSeek score 53.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6669
variable_sites: 165
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGATCAATGGAGGAACACCC	AGAAGATATCAATGAACTTCTATGA	50.0	28.0	57.2	53.6	200 bp	1.0	45.6
ycf2_p2	TGATCAATGGAGGAACACCC	AAGAAGATATCAATGAACTTCTATGA	50.0	26.9	57.2	54.3	201 bp	1.0	45.6
ycf2_p3	TGATCAATGGAGGAACACCC	AGAAGATATCAATGAACTTCTATGAA	50.0	26.9	57.2	54.3	200 bp	1.0	45.6
ycf2_p4	TTGATCAATGGAGGAACACCC	AGAAGATATCAATGAACTTCTATGA	47.6	28.0	57.9	53.6	201 bp	1.0	45.6
ycf2_p5	TGATCAATGGAGGAACACCC	AAAAGAAGATATCAATGAACTTCT	50.0	25.0	57.2	52.4	203 bp	1.0	45.5