Hotspot

clpP1

LSC · 70398-72501 · 2104 bp

Back to Hygrophila

Pi 0.0173

MarkerSeek score 56.70

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2104
variable_sites: 34
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TTGCTCAGGATCGAAACGAG	TCATACAAAGCCATCGAGCC	50.0	50.0	58.0	58.0	2791-2948 bp	1.0	85.2
clpP1_p2	TGACATTTCGAATCGGCTGT	TCATACAAAGCCATCGAGCC	45.0	50.0	57.9	58.0	2706-2863 bp	1.0	84.5
clpP1_p3	TGTGACATTTCGAATCGGCT	TCATACAAAGCCATCGAGCC	45.0	50.0	57.9	58.0	2708-2865 bp	1.0	84.5
clpP1_p4	TTGCTCAGGATCGAAACGAG	ATACCAAGGCAAACCCATGG	50.0	50.0	58.0	58.1	2687-2844 bp	1.0	84.4
clpP1_p5	TGACATTTCGAATCGGCTGT	ACGAACATACCCTGTCTCCA	45.0	50.0	57.9	58.1	2769-2926 bp	1.0	84.3