Hotspot

ndhF-rpl32

SSC · 111980-112526 · 547 bp

Back to Hygrophila

Pi 0.0752
MarkerSeek score 81.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
547
variable_sites
40
pi
0.0752
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGCAGAACGAAAGGT TGATGCAGAAATGCGTTTTT 50.0 35.0 58.1 55.1 632-670 bp 1.0 62.3
ndhF-rpl32_p2 CTGGAAGCAGAACGAAAGGT TTGATGCAGAAATGCGTTTT 50.0 35.0 58.1 55.1 633-671 bp 1.0 62.3
ndhF-rpl32_p3 CTGGAAGCAGAACGAAAGGT TTTGATGCAGAAATGCGTTT 50.0 35.0 58.1 55.1 634-672 bp 1.0 62.3
ndhF-rpl32_p4 CTGGAAGCAGAACGAAAGGT TTTTGATGCAGAAATGCGTT 50.0 35.0 58.1 55.1 635-673 bp 1.0 62.3
ndhF-rpl32_p5 CTGGAAGCAGAACGAAAGGT TTTTTGATGCAGAAATGCGT 50.0 35.0 58.1 55.1 636-674 bp 1.0 62.3