Hotspot

psaJ-rpl33

LSC · 67698-68053 · 356 bp

Back to Hygrophila

Pi 0.0538

MarkerSeek score 74.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 19
pi: 0.0538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CTATGGTTCGGGGCTTTAGC	ACCTTCTTAACACCGGTTCG	55.0	50.0	58.4	57.8	520 bp	1.0	78.9
psaJ-rpl33_p2	GCTATGGTTCGGGGCTTTAG	ACCTTCTTAACACCGGTTCG	55.0	50.0	58.4	57.8	521 bp	1.0	78.9
psaJ-rpl33_p3	CTATGGTTCGGGGCTTTAGC	AAAATTACCGTTACGCGTGC	55.0	45.0	58.4	57.8	481 bp	1.0	78.6
psaJ-rpl33_p4	GCTATGGTTCGGGGCTTTAG	AAAATTACCGTTACGCGTGC	55.0	45.0	58.4	57.8	482 bp	1.0	78.6
psaJ-rpl33_p5	CTATGGTTCGGGGCTTTAGC	CGCGTGCATCTTTCCTCTTA	55.0	50.0	58.4	58.4	468 bp	1.0	77.6