Hotspot

psbL-psbF

LSC · 65221-65244 · 24 bp

Back to Hygrophila

Pi 0.0833

MarkerSeek score 85.40

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 24
variable_sites: 2
pi: 0.0833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	CACGGACTAGCTGTACCAAC	45.0	55.0	58.0	58.0	116 bp	1.0	81.0
psbL-psbF_p2	TCGTTCGGGTTTGATTGTGT	ACTAGCTGTACCAACCGTCT	45.0	50.0	58.0	58.1	111 bp	1.0	80.5
psbL-psbF_p3	TCGTTCGGGTTTGATTGTGT	TCACGGACTAGCTGTACCAA	45.0	50.0	58.0	58.1	117 bp	1.0	80.3
psbL-psbF_p4	TCGTTCGGGTTTGATTGTGT	TTCACGGACTAGCTGTACCA	45.0	50.0	58.0	58.1	118 bp	1.0	80.3
psbL-psbF_p5	TCGTTCGGGTTTGATTGTGT	AGTACGATGGTTGGCTGTTC	45.0	50.0	58.0	57.9	135 bp	1.0	80.1