Hotspot

ndhC-trnV-UAC

LSC · 50861-52028 · 1168 bp

Back to Hygrophila

Pi 0.0416

MarkerSeek score 75.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1168
variable_sites: 46
pi: 0.0416
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	27.3	50.0	52.7	57.8	1171-1224 bp	1.0	47.1
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAAAAACA	GAAGGTCTACGGTTCGAGTC	27.3	55.0	52.7	57.5	1170-1223 bp	1.0	47.1
ndhC-trnV-UAC_p3	ATCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	26.1	50.0	53.3	57.8	1172-1225 bp	1.0	47.1
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAAAAACAT	AGAAGGTCTACGGTTCGAGT	26.1	50.0	53.3	57.8	1171-1224 bp	1.0	47.1
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAAAAACA	GTTCGAGTCCGTATAGCCCT	27.3	55.0	52.7	58.7	1159-1212 bp	1.0	47.1