Hotspot

trnF-GAA-ndhJ

LSC · 48493-49187 · 695 bp

Back to Hygrophila

Pi 0.0287

MarkerSeek score 75.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 19
pi: 0.0287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGCTAGGATGGC	55.0	50.0	58.0	57.9	822-841 bp	1.0	83.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGCTAGGATGGC	50.0	50.0	58.1	57.9	786-805 bp	1.0	83.7
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TGAAAGTTGGCTAGGATGGC	55.0	50.0	57.9	57.9	810-829 bp	1.0	82.9
trnF-GAA-ndhJ_p4	ATGAGGGTGGGATGCTACAT	TGAAAGTTGGCTAGGATGGC	50.0	50.0	58.2	57.9	853-872 bp	1.0	82.3
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCTGAAAGTTGGCTAGGAT	55.0	50.0	58.0	57.6	825-844 bp	1.0	81.5