Hotspot

psaA-pafI

LSC · 42175-42833 · 659 bp

Back to Hygrophila

Pi 0.0415

MarkerSeek score 78.20

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 26
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TTTTACTTCTGGTTCCGGCG	AGCCCTTACCCCTGGTAATT	50.0	50.0	58.5	58.0	759-763 bp	1.0	82.3
psaA-pafI_p2	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	790-794 bp	1.0	81.8
psaA-pafI_p3	TTTTACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	719-723 bp	1.0	81.4
psaA-pafI_p4	TTTTACTTCTGGTTCCGGCG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	723-727 bp	1.0	81.1
psaA-pafI_p5	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	786-790 bp	1.0	80.0