Hotspot

trnS-GCU-trnG-UCC

LSC · 8171-8975 · 805 bp

Back to Hygrophila

Pi 0.0429
MarkerSeek score 72.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
805
variable_sites
25
pi
0.0429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTACACCCGC 50.0 50.0 58.0 57.0 660-860 bp 1.0 78.3
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTACACCCGC 50.0 50.0 58.0 57.0 659-859 bp 1.0 78.3
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TACCACTAAACTACACCCGC 50.0 50.0 58.2 57.0 714-914 bp 1.0 77.0
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA TTACCACTAAACTACACCCGC 50.0 47.6 58.0 57.7 661-861 bp 1.0 76.1
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC TTACCACTAAACTACACCCGC 50.0 47.6 58.0 57.7 660-860 bp 1.0 76.0