Hotspot

trnW-CCA-trnP-UGG

LSC · 67538-67739 · 202 bp

Back to Hydrostachys

Pi 0.101

MarkerSeek score 82.20

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 25
pi: 0.101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	251-269 bp	1.0	90.5
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	253-271 bp	1.0	90.4
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	254-272 bp	1.0	90.4
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	264-282 bp	1.0	90.2
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	265-283 bp	1.0	90.2