Hotspot

trnT-UGU-trnL-UAA

LSC · 47749-48679 · 931 bp

Back to Hydrostachys

Pi 0.0484
MarkerSeek score 80.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
931
variable_sites
64
pi
0.0484
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AATGAAGCGAAGTCGACGAA ATAAAGGGGGCTTTTTGGGG 45.0 50.0 57.9 58.0 1971-1981 bp 0.666667 69.8
trnT-UGU-trnL-UAA_p2 CTTGTCTGGCGATACGAACA ATAAAGGGGGCTTTTTGGGG 50.0 50.0 58.0 58.0 2296 bp 0.333333 53.0
trnT-UGU-trnL-UAA_p3 CTTGTCTGGCGATACGAACA GGCTTTTTGGGGATAGAGGG 50.0 55.0 58.0 57.9 2288 bp 0.333333 52.7
trnT-UGU-trnL-UAA_p4 CTTGTCTGGCGATACGAACA GGGCTTTTTGGGGATAGAGG 50.0 55.0 58.0 57.9 2289 bp 0.333333 52.7
trnT-UGU-trnL-UAA_p5 CTTGTCTGGCGATACGAACA GGGGCTTTTTGGGGATAGAG 50.0 55.0 58.0 57.9 2290 bp 0.333333 52.7