Hotspot

psaA-ycf3

LSC · 42187-43335 · 1149 bp

Back to Hydrostachys

Pi 0.0413

MarkerSeek score 78.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 69
pi: 0.0413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	GACCAGGCTGCTGAGTATTG	50.0	55.0	58.5	58.3	1281-1341 bp	1.0	80.4
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	AAATTGGGGAGGCTTGGTTC	50.0	50.0	58.5	58.4	1301-1361 bp	1.0	80.2
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	GAAATTGGGGAGGCTTGGTT	50.0	50.0	58.5	58.4	1302-1362 bp	1.0	80.2
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	CAGGCTGCTGAGTATTGGAA	50.0	50.0	58.5	57.6	1278-1338 bp	1.0	79.9
psaA-ycf3_p5	TTTACTTCTGGTTCCGGCG	GACCAGGCTGCTGAGTATTG	52.6	55.0	57.8	58.3	1280-1340 bp	1.0	74.6