Hotspot

trnS-GCU-trnG-UCC

LSC · 7822-8496 · 675 bp

Back to Hydrostachys

Pi 0.0924

MarkerSeek score 86.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 83
pi: 0.0924
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	744-899 bp	1.0	63.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	743-898 bp	1.0	63.7
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	747-902 bp	1.0	62.6
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	746-901 bp	1.0	62.6
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	745-900 bp	1.0	62.6