Hotspot

orf136

Genome · 136279-136977 · 699 bp

Back to Hydrolithon

Pi 0.0229

MarkerSeek score 57.10

Primer available yes

Label orf136

Feature Metadata

label_name: orf136
feature_type: gene
region: Genome
length_bp: 699
variable_sites: 16
pi: 0.0229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf136_p1	AAACCTGAATGGGAAGCTAA	CCAAGCTAGTAATGCGGGTT	40.0	50.0	54.5	58.0	1045 bp	1.0	53.4
orf136_p2	AACCTGAATGGGAAGCTAAA	CCAAGCTAGTAATGCGGGTT	40.0	50.0	54.5	58.0	1044 bp	1.0	53.4
orf136_p3	AAACCTGAATGGGAAGCTAA	AGTAATGCGGGTTCGATTCC	40.0	50.0	54.5	58.1	1038 bp	1.0	53.4
orf136_p4	AACCTGAATGGGAAGCTAAA	AGTAATGCGGGTTCGATTCC	40.0	50.0	54.5	58.1	1037 bp	1.0	53.4
orf136_p5	AACCTGAATGGGAAGCTAAA	AAGCTAGTAATGCGGGTTCG	40.0	50.0	54.5	58.1	1042 bp	1.0	53.2