Hotspot

cpcA-pgmA

Genome · 161283-161423 · 141 bp

Back to Hydrolithon

Pi 0.0993

MarkerSeek score 78.00

Primer available yes

Label cpcA-pgmA

Feature Metadata

label_name: cpcA-pgmA
feature_type: igs
region: Genome
length_bp: 141
variable_sites: 14
pi: 0.0993
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cpcA-pgmA_p1	GATTATCCGGACAAGCAGCT	AACCCAATCCGTCAAGAATT	50.0	40.0	57.8	55.2	267 bp	1.0	60.0
cpcA-pgmA_p2	TGGATTATCCGGACAAGCAG	AACCCAATCCGTCAAGAATT	50.0	40.0	57.4	55.2	269 bp	1.0	57.0
cpcA-pgmA_p3	GGATTATCCGGACAAGCAGC	AACCCAATCCGTCAAGAATT	55.0	40.0	58.8	55.2	268 bp	1.0	55.8
cpcA-pgmA_p4	CGGACAAGCAGCTATCGAAG	AACCCAATCCGTCAAGAATT	55.0	40.0	58.8	55.2	260 bp	1.0	55.7
cpcA-pgmA_p5	GATTATCCGGACAAGCAGCT	TCATGTTATAACCCAATCCGTCA	50.0	39.1	57.8	57.6	276 bp	1.0	55.2