Hotspot

rpl19-trnV-GAC

Genome · 52544-52557 · 14 bp

Back to Hydrolithon

Pi 0.0714
MarkerSeek score 75.70
Primer available yes
Label rpl19-trnV-GAC

Feature Metadata

label_name
rpl19-trnV-GAC
feature_type
igs
region
Genome
length_bp
14
variable_sites
1
pi
0.0714
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl19-trnV-GAC_p1 AGAGTAGGTAAAGCTACAAGA GGATTCGAACCAGTGGCTTT 38.1 50.0 53.1 58.5 122 bp 1.0 40.7
rpl19-trnV-GAC_p2 AGAGTAGGTAAAGCTACAAGAT GGATTCGAACCAGTGGCTTT 36.4 50.0 53.7 58.5 122 bp 1.0 40.7
rpl19-trnV-GAC_p3 AGAGTAGGTAAAGCTACAAGATT GGATTCGAACCAGTGGCTTT 34.8 50.0 54.5 58.5 122 bp 1.0 40.7
rpl19-trnV-GAC_p4 AGAGTAGGTAAAGCTACAAGA GGATTCGAACCAGTGGCTT 38.1 52.6 53.1 57.8 122 bp 1.0 40.7
rpl19-trnV-GAC_p5 AGAGTAGGTAAAGCTACAAGA GATTCGAACCAGTGGCTTTT 38.1 45.0 53.1 56.6 121 bp 1.0 40.7