Hotspot

dnaB-trnF-GAA

Genome · 49325-49377 · 53 bp

Back to Hydrolithon

Pi 0.0755

MarkerSeek score 75.70

Primer available yes

Label dnaB-trnF-GAA

Feature Metadata

label_name: dnaB-trnF-GAA
feature_type: igs
region: Genome
length_bp: 53
variable_sites: 4
pi: 0.0755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB-trnF-GAA_p1	ACAGGCTCATTCCAACTGTT	TTTGAACTGGTGACACGAGG	45.0	50.0	57.3	58.1	196 bp	1.0	75.9
dnaB-trnF-GAA_p2	ACAGGCTCATTCCAACTGTT	TCTACCGACTGAGCTATCCC	45.0	55.0	57.3	57.7	160 bp	1.0	74.5
dnaB-trnF-GAA_p3	ACAGGCTCATTCCAACTGTTT	TTTGAACTGGTGACACGAGG	42.9	50.0	58.1	58.1	196 bp	1.0	72.8
dnaB-trnF-GAA_p4	ACAGGCTCATTCCAACTGTT	GAACTGGTGACACGAGGATT	45.0	50.0	57.3	57.5	193 bp	1.0	72.7
dnaB-trnF-GAA_p5	ACAGGCTCATTCCAACTGTTT	TCTACCGACTGAGCTATCCC	42.9	55.0	58.1	57.7	160 bp	1.0	71.4