Hotspot

rpl11-trnW-CCA

Genome · 44896-45509 · 614 bp

Back to Hydrolithon

Pi 0.0312

MarkerSeek score 67.80

Primer available yes

Label rpl11-trnW-CCA

Feature Metadata

label_name: rpl11-trnW-CCA
feature_type: igs
region: Genome
length_bp: 614
variable_sites: 17
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl11-trnW-CCA_p1	ACCTGCATTTAAAGCTAACTTGAC	CCTAATGCCGAGGGTTCAAA	37.5	50.0	58.0	57.9	626-696 bp	1.0	53.0
rpl11-trnW-CCA_p2	ACCTGCATTTAAAGCTAACTTGAC	TCCAAAACCTAATGCCGAGG	37.5	50.0	58.0	57.9	633-703 bp	1.0	52.9
rpl11-trnW-CCA_p3	ACCTGCATTTAAAGCTAACTTGAC	AAACCTAATGCCGAGGGTTC	37.5	50.0	58.0	57.9	629-699 bp	1.0	52.9
rpl11-trnW-CCA_p4	ACCTGCATTTAAAGCTAACTTGAC	CAAAACCTAATGCCGAGGGT	37.5	50.0	58.0	58.2	631-701 bp	1.0	52.6
rpl11-trnW-CCA_p5	CCTGCATTTAAAGCTAACTTGACT	CCTAATGCCGAGGGTTCAAA	37.5	50.0	57.7	57.9	625-695 bp	1.0	51.0