Hotspot

psaJ-psaF

Genome · 18036-18069 · 34 bp

Back to Hydrolithon

Pi 0.176

MarkerSeek score 78.60

Primer available yes

Label psaJ-psaF

Feature Metadata

label_name: psaJ-psaF
feature_type: igs
region: Genome
length_bp: 34
variable_sites: 6
pi: 0.176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-psaF_p1	ACCAGCTGTAAAAGTTAGCCA	CCATTAGCAGCTTGGCAAGA	42.9	50.0	57.5	58.5	187 bp	1.0	66.3
psaJ-psaF_p2	AACCAGCTGTAAAAGTTAGCCA	CCATTAGCAGCTTGGCAAGA	40.9	50.0	58.2	58.5	188 bp	1.0	60.9
psaJ-psaF_p3	ACCAGCTGTAAAAGTTAGCCAA	CCATTAGCAGCTTGGCAAGA	40.9	50.0	58.2	58.5	187 bp	1.0	60.9
psaJ-psaF_p4	ACCAGCTGTAAAAGTTAGCCA	CATTAGCAGCTTGGCAAGAA	42.9	45.0	57.5	56.7	186 bp	1.0	60.1
psaJ-psaF_p5	ACCAGCTGTAAAAGTTAGCCA	TGGCAAGAATTTACTAGCGGT	42.9	42.9	57.5	57.7	175 bp	1.0	60.0