Hotspot

rpl21-rpl32

Genome · 8129-8984 · 856 bp

Back to Hydrolithon

Pi 0.0245

MarkerSeek score 58.10

Primer available yes

Label rpl21-rpl32

Feature Metadata

label_name: rpl21-rpl32
feature_type: igs
region: Genome
length_bp: 856
variable_sites: 21
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21-rpl32_p1	GACCTCTTTTGGATCGTGCA	CAGCAGCTTCTTGGGCTATT	50.0	50.0	58.2	58.2	1455 bp	0.5	56.5
rpl21-rpl32_p2	TGACCTCTTTTGGATCGTGC	CAGCAGCTTCTTGGGCTATT	50.0	50.0	58.2	58.2	1456 bp	0.5	56.5
rpl21-rpl32_p3	GACCTCTTTTGGATCGTGCA	TGATTCCTGTTTTGGCAGCA	50.0	45.0	58.2	58.3	1470 bp	0.5	56.1
rpl21-rpl32_p4	TGACCTCTTTTGGATCGTGC	TGATTCCTGTTTTGGCAGCA	50.0	45.0	58.2	58.3	1471 bp	0.5	56.1
rpl21-rpl32_p5	GACCTCTTTTGGATCGTGCA	GATTCCTGTTTTGGCAGCAG	50.0	50.0	58.2	57.6	1469 bp	0.5	55.6