Hotspot

trnN-GUU-trnL-UAG

Genome · 4492-4608 · 117 bp

Back to Hydrolithon

Pi 0.0598
MarkerSeek score 74.40
Primer available yes
Label trnN-GUU-trnL-UAG

Feature Metadata

label_name
trnN-GUU-trnL-UAG
feature_type
igs
region
Genome
length_bp
117
variable_sites
7
pi
0.0598
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnL-UAG_p1 ACTCCCCAGGAAGGATTTGA TACCAATTTCACCACGTCCG 50.0 50.0 57.9 58.2 211 bp 1.0 80.6
trnN-GUU-trnL-UAG_p2 CCGATTGCTCTACCACTGAG TACCAATTTCACCACGTCCG 55.0 50.0 57.8 58.2 168 bp 1.0 80.0
trnN-GUU-trnL-UAG_p3 CCCCAGGAAGGATTTGAACC TACCAATTTCACCACGTCCG 55.0 50.0 58.1 58.2 208 bp 1.0 79.9
trnN-GUU-trnL-UAG_p4 ACTCCCCAGGAAGGATTTGA AATGCGGACAGAGAGATTCG 50.0 50.0 57.9 57.8 275 bp 1.0 79.8
trnN-GUU-trnL-UAG_p5 CCCCAGGAAGGATTTGAACC AATGCGGACAGAGAGATTCG 55.0 50.0 58.1 57.8 272 bp 1.0 79.2