Hotspot

rps6-ycf60

Genome · 389-418 · 30 bp

Back to Hydrolithon

Pi 0.1

MarkerSeek score 76.50

Primer available yes

Label rps6-ycf60

Feature Metadata

label_name: rps6-ycf60
feature_type: igs
region: Genome
length_bp: 30
variable_sites: 3
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps6-ycf60_p1	ACTTCAGTTATATCAGGTTTTACA	TCCCGTAATTTCACAAGCTGT	29.2	42.9	53.7	57.9	135 bp	1.0	41.8
rps6-ycf60_p2	ACTTCAGTTATATCAGGTTTTACAA	TCCCGTAATTTCACAAGCTGT	28.0	42.9	54.5	57.9	135 bp	1.0	41.8
rps6-ycf60_p3	ACTTCAGTTATATCAGGTTTTACA	CCCGTAATTTCACAAGCTGTC	29.2	47.6	53.7	57.5	134 bp	1.0	41.8
rps6-ycf60_p4	GAGTATCTACTTCAGTTATATCAGGT	TCCCGTAATTTCACAAGCTGT	34.6	42.9	55.4	57.9	143 bp	1.0	41.7
rps6-ycf60_p5	TCTACTTCAGTTATATCAGGTTT	TCCCGTAATTTCACAAGCTGT	30.4	42.9	52.3	57.9	138 bp	1.0	41.7