Hotspot

ycf1

SSC · 123436-128934 · 5499 bp

Back to Hydrocotyle

Pi 0.0144

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5499
variable_sites: 327
pi: 0.0144
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAGGGACATCCTTCTTTGT	GCAGAATACCGTCACCCATT	50.0	50.0	57.8	58.0	501-534 bp	1.0	90.0
ycf1_p2	GCAGGGACATCCTTCTTTGT	AGGGCCCTCTTATCTCTTCC	50.0	55.0	57.8	57.9	1103-1136 bp	1.0	84.2
ycf1_p3	GCAGGGACATCCTTCTTTGT	GGGCCCTCTTATCTCTTCCT	50.0	55.0	57.8	57.9	1102-1135 bp	1.0	84.2
ycf1_p4	GCAGGGACATCCTTCTTTGT	AAGGTATCAGCAGCAACAGG	50.0	50.0	57.8	57.9	1036-1069 bp	1.0	84.0
ycf1_p5	TAGTATGGGCAGGGACATCC	GCAGAATACCGTCACCCATT	55.0	50.0	58.3	58.0	509-542 bp	0.777778	76.8