Hotspot

rpl22

LSC · 83405-83899 · 495 bp

Back to Hydrocotyle

Pi 0.00958

MarkerSeek score 46.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 495
variable_sites: 20
pi: 0.00958
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	ACCAAGTCTGAAACCGAGTG	TGCTTATCATTTATTAGTAGGAGACA	50.0	30.8	57.8	55.8	546-557 bp	0.888889	41.2
rpl22_p2	CCAAGTCTGAAACCGAGTGG	TGCTTATCATTTATTAGTAGGAGACA	55.0	30.8	58.5	55.8	545-556 bp	0.888889	41.2
rpl22_p3	CAAGTCTGAAACCGAGTGGA	TGCTTATCATTTATTAGTAGGAGACA	50.0	30.8	57.5	55.8	544-555 bp	0.888889	41.2
rpl22_p4	ACCAAGTCTGAAACCGAGTG	TGCTTATCATTTATTAGTAGGAGACAA	50.0	29.6	57.8	56.4	546-557 bp	0.888889	41.2
rpl22_p5	GTTGTGCAAACCAAAGGGAA	TGCTTATCATTTATTAGTAGGAGACA	45.0	30.8	57.3	55.8	583-594 bp	0.888889	40.8