Hotspot

rpl16

LSC · 81216-82613 · 1398 bp

Back to Hydrocotyle

Pi 0.0135

MarkerSeek score 55.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1398
variable_sites: 84
pi: 0.0135
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCGACGATTGCTAGCTCCTA	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.0	58.0	1911-1979 bp	1.0	86.5
rpl16_p2	TCGACGATTGCTAGCTCCTA	TGACGGAAAAGAAATTGCGC	50.0	45.0	58.0	58.0	1888-1956 bp	1.0	86.4
rpl16_p3	GCAATTGCACCAAAAACCCT	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.0	58.0	2153-2162 bp	0.611111	65.3
rpl16_p4	GCAATTGCACCAAAAACCCT	TGACGGAAAAGAAATTGCGC	45.0	45.0	58.0	58.0	2130-2139 bp	0.611111	65.1
rpl16_p5	GCAATTGCACCAAAAACCCT	TCCAGTGTTGAGGGAATTGC	45.0	50.0	58.0	58.1	2471-2480 bp	0.611111	64.7