Hotspot

rpl32-trnL-UAG

SSC · 113041-113824 · 784 bp

Back to Hydrocotyle

Pi 0.0174
MarkerSeek score 59.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
784
variable_sites
64
pi
0.0174
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAGTCA AGCGTGTCTACCGATTTCAC 36.4 50.0 57.1 58.0 799-851 bp 1.0 67.0
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAGTCA CAGCGTGTCTACCGATTTCA 36.4 50.0 57.1 58.0 800-852 bp 1.0 67.0
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAGTCA CTCTGGCACTGCTTCCTAAG 36.4 55.0 57.1 58.0 822-874 bp 1.0 66.7
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAGTCA CTGGCACTGCTTCCTAAGAG 36.4 55.0 57.1 58.0 820-872 bp 1.0 66.7
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAGTCA TCCTAAGAGCAGCGTGTCTA 36.4 50.0 57.1 57.9 809-861 bp 1.0 66.0