Hotspot

rpl16-rps3

LSC · 82614-82760 · 147 bp

Back to Hydrocotyle

Pi 0.0485

MarkerSeek score 65.90

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 20
pi: 0.0485
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	CCTGCAAACCATTCGAGCTA	45.0	50.0	57.6	58.3	278-279 bp	1.0	80.8
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	CCTGCAAACCATTCGAGCTA	45.0	50.0	57.1	58.3	273-274 bp	1.0	77.0
rpl16-rps3_p3	ACCAACGAGTCACACACTAAG	CCTGCAAACCATTCGAGCTA	47.6	50.0	57.9	58.3	276-277 bp	1.0	75.2
rpl16-rps3_p4	ACGAGTCACACACTAAGCAT	CCTGCAAACCATTCGAGCTA	45.0	50.0	56.9	58.3	272-273 bp	1.0	75.2
rpl16-rps3_p5	AAAACCAACGAGTCACACAC	CCTGCAAACCATTCGAGCTA	45.0	50.0	56.8	58.3	279-280 bp	1.0	74.3