Hotspot

psbH-petB

LSC · 74811-74933 · 123 bp

Back to Hydrocotyle

Pi 0.0388

MarkerSeek score 59.30

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 18
pi: 0.0388
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TCTTCCGTTTTATTGGATGA	ACGAAGTCGAACCACCTTTA	35.0	45.0	52.2	56.8	243-244 bp	0.777778	36.4
psbH-petB_p2	TCTTCCGTTTTATTGGATGAA	ACGAAGTCGAACCACCTTTA	33.3	45.0	53.1	56.8	243-244 bp	0.777778	36.4
psbH-petB_p3	TTCTTCCGTTTTATTGGATGA	ACGAAGTCGAACCACCTTTA	33.3	45.0	53.1	56.8	244-245 bp	0.777778	36.4
psbH-petB_p4	TCTTCCGTTTTATTGGATGAAA	ACGAAGTCGAACCACCTTTA	31.8	45.0	54.0	56.8	243-244 bp	0.777778	36.4
psbH-petB_p5	TTCTTCCGTTTTATTGGATGAA	ACGAAGTCGAACCACCTTTA	31.8	45.0	54.0	56.8	244-245 bp	0.777778	36.4