Hotspot

ndhC-trnV-UAC

LSC · 51336-52328 · 993 bp

Back to Hydrocotyle

Pi 0.0153

MarkerSeek score 55.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 58
pi: 0.0153
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	452-1037 bp	1.0	48.9
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.9	57.8	464-1049 bp	0.944444	46.4
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.9	57.5	463-1048 bp	0.944444	46.4
ndhC-trnV-UAC_p4	ATCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	465-1050 bp	0.944444	46.4
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	464-1049 bp	0.944444	46.4