Hotspot

ycf3-trnS-GGA

LSC · 44950-45814 · 865 bp

Back to Hydrocotyle

Pi 0.0235

MarkerSeek score 68.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 67
pi: 0.0235
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCTCGATCTGGGC	CCCTCGGTAAACAAAAGCCT	52.4	50.0	58.3	58.1	920-967 bp	1.0	78.5
ycf3-trnS-GGA_p2	CCATTTATCCTCGATCTGGGC	ACGGAAAGAGAGGGATTCGA	52.4	50.0	58.3	58.2	941-988 bp	1.0	77.9
ycf3-trnS-GGA_p3	CCATTTATCCTCGATCTGGGC	TTCCAATGCTACGCCTTCAA	52.4	45.0	58.3	57.8	891-938 bp	1.0	77.9
ycf3-trnS-GGA_p4	CATTTATCCTCGATCTGGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.6	58.1	919-966 bp	1.0	77.6
ycf3-trnS-GGA_p5	CCATTTATCCTCGATCTGGGC	AGTTCCAATGCTACGCCTTC	52.4	50.0	58.3	58.3	893-940 bp	1.0	77.3