Hotspot

trnG-GCC-trnfM-CAU

LSC · 36853-37048 · 196 bp

Back to Hydrocotyle

Pi 0.0179

MarkerSeek score 60.10

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 17
pi: 0.0179
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	248-281 bp	1.0	87.3
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	284-317 bp	1.0	86.9
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	242-275 bp	1.0	86.9
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	241-274 bp	1.0	86.4
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	246-279 bp	1.0	86.3