Hotspot

petN-psbM

LSC · 28812-29907 · 1096 bp

Back to Hydrocotyle

Pi 0.0152

MarkerSeek score 56.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 71
pi: 0.0152
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.6	57.2	923-1197 bp	1.0	71.9
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCTT	50.0	40.9	57.6	57.9	923-1197 bp	1.0	69.4
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGC	50.0	45.0	57.6	55.7	923-1197 bp	1.0	67.5
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GTTCCTACTGCTTTTCTGCT	50.0	45.0	57.6	55.7	922-1196 bp	1.0	67.5
petN-psbM_p5	TGGGGAAGAAGTGGACTCTA	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.0	57.2	922-1196 bp	1.0	66.6