Hotspot

rpoB-trnC-GCA

LSC · 27210-28297 · 1088 bp

Back to Hydrocotyle

Pi 0.0156

MarkerSeek score 62.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 71
pi: 0.0156
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1114-1151 bp	1.0	85.3
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1154-1191 bp	1.0	85.0
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCTCGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1136-1173 bp	1.0	82.9
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCTCGGA	CCTTACCACTCGGCCATATC	50.0	55.0	57.8	57.5	1115-1152 bp	1.0	82.2
rpoB-trnC-GCA_p5	TCCCTCATTTCCATCTCGGA	TTTGTTGATCAGGCGACACC	50.0	50.0	57.8	58.8	1173-1210 bp	1.0	79.4