Hotspot

atpH-atpI

LSC · 13698-14899 · 1202 bp

Back to Hydrocotyle

Pi 0.0134

MarkerSeek score 55.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1202
variable_sites: 65
pi: 0.0134
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGC	45.0	55.0	58.0	58.9	1202-1263 bp	1.0	81.2
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TAGGTGAATCCATGGAGGGC	50.0	55.0	57.9	58.9	1258-1319 bp	1.0	80.5
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGC	45.0	55.0	58.2	58.9	1203-1264 bp	1.0	80.0
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	TAGGTGAATCCATGGAGGGC	55.0	55.0	58.2	58.9	1262-1323 bp	1.0	79.8
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	TAGGTGAATCCATGGAGGGC	50.0	55.0	57.8	58.9	1232-1293 bp	0.944444	77.6