Hotspot

psbK-psbI

LSC · 7955-8359 · 405 bp

Back to Hydrocotyle

Pi 0.0165

MarkerSeek score 55.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 27
pi: 0.0165
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGAAAAGTTTGAGAGTAAGCA	50.0	36.4	57.9	56.2	452-479 bp	1.0	56.3
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	449-476 bp	1.0	54.3
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	450-477 bp	1.0	54.3
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	451-478 bp	1.0	54.3
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACGAAAAGTTTGAGAGTAAGCA	50.0	34.8	57.9	56.9	453-480 bp	1.0	53.8