Hotspot

rps16-trnQ-UUG

LSC · 6159-7331 · 1173 bp

Back to Hydrocotyle

Pi 0.0154

MarkerSeek score 63.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 84
pi: 0.0154
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1246-1415 bp	1.0	87.2
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1261-1430 bp	1.0	85.4
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1260-1429 bp	1.0	85.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	1267-1436 bp	1.0	83.5
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1250-1419 bp	1.0	82.5