Hotspot

ycf1

IRb · 107580-113999 · 6420 bp

Back to Hydrocharis

Pi 0.353

MarkerSeek score 66.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 6420
variable_sites: 1175
pi: 0.353
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.48
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGGCAAAGACAAAGACAGG	ACGTAGCATATACAGCCCCT	50.0	50.0	58.0	58.0	968 bp	0.333333	53.1
ycf1_p2	AAACGCAAACGCAAAGACAA	ACGTAGCATATACAGCCCCT	40.0	50.0	58.0	58.0	939 bp	0.333333	53.1
ycf1_p3	AAAGACAAACGCAAACGCAA	ACGTAGCATATACAGCCCCT	40.0	50.0	58.0	58.0	945 bp	0.333333	53.1
ycf1_p4	AACGCAAACGCAAAGACAAA	ACGTAGCATATACAGCCCCT	40.0	50.0	58.0	58.0	938 bp	0.333333	53.1
ycf1_p5	AAGACAAACGCAAACGCAAA	ACGTAGCATATACAGCCCCT	40.0	50.0	58.0	58.0	944 bp	0.333333	53.1