Hotspot

accD

LSC · 56912-58609 · 1698 bp

Back to Hydrocharis

Pi 0.0815
MarkerSeek score 83.10
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1698
variable_sites
195
pi
0.0815
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TGTCTTTCTCGTTGCCTGTT ATGAGATTTGGTCCCCCTCA 45.0 50.0 57.7 58.0 2551 bp 0.333333 50.6
accD_p2 TGTCTTTCTCGTTGCCTGTT TGAGATTTGGTCCCCCTCAT 45.0 50.0 57.7 58.0 2550 bp 0.333333 50.6
accD_p3 TTGTCTTTCTCGTTGCCTGT ATGAGATTTGGTCCCCCTCA 45.0 50.0 57.7 58.0 2552 bp 0.333333 50.6
accD_p4 TTGTCTTTCTCGTTGCCTGT TGAGATTTGGTCCCCCTCAT 45.0 50.0 57.7 58.0 2551 bp 0.333333 50.6
accD_p5 TGTCTTTCTCGTTGCCTGTT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.7 58.0 2487 bp 0.333333 50.5