Hotspot

trnN-GUU-ycf1

IRb · 107251-107579 · 329 bp

Back to Hydrocharis

Pi 0.473

MarkerSeek score 72.60

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 329
variable_sites: 161
pi: 0.473
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GGAACAGATTCGGTGGAGAC	GGCGAATGGCATTCTTCCTA	55.0	50.0	58.0	58.0	1699 bp	0.333333	53.0
trnN-GUU-ycf1_p2	GGAACAGATTCGGTGGAGAC	CCTTCTTCCATAACCCGAGC	55.0	55.0	58.0	58.0	1164 bp	0.333333	52.9
trnN-GUU-ycf1_p3	GGAACAGATTCGGTGGAGAC	ATTTTAGGCGGATGTGGGAC	55.0	50.0	58.0	57.9	1598 bp	0.333333	52.8
trnN-GUU-ycf1_p4	GGAACAGATTCGGTGGAGAC	TATGAACATCACGAGCTGCC	55.0	50.0	58.0	58.1	1225 bp	0.333333	52.8
trnN-GUU-ycf1_p5	ATTCGAACCTACGGCCAATC	GGCGAATGGCATTCTTCCTA	50.0	50.0	58.1	58.0	1072 bp	0.333333	52.7