Hotspot

psbM-trnD-GUC

LSC · 30018-30836 · 819 bp

Back to Hydrocharis

Pi 0.0493

MarkerSeek score 84.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 58
pi: 0.0493
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGAACTAGAATAAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	37.5	50.0	57.4	58.5	937-1066 bp	1.0	47.3
psbM-trnD-GUC_p2	AGTAAAAAGGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	38.1	50.0	54.5	58.5	951-1080 bp	1.0	47.2
psbM-trnD-GUC_p3	AAGTAAAAAGGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	36.4	50.0	55.3	58.5	952-1081 bp	1.0	47.2
psbM-trnD-GUC_p4	AAAAGGCAGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	40.0	50.0	53.3	58.5	947-1076 bp	1.0	47.2
psbM-trnD-GUC_p5	AAAGGCAGTAGGAACTAGAA	TTCAATTGGTCAGAGCACCG	40.0	50.0	53.3	58.5	946-1075 bp	1.0	47.2