Hotspot

trnC-GCA-petN

LSC · 27806-28493 · 688 bp

Back to Hydrocharis

Pi 0.0576

MarkerSeek score 87.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 57
pi: 0.0576
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	CTCCTCCCCCATACTACGAG	50.0	60.0	58.5	58.1	790-826 bp	1.0	85.2
trnC-GCA-petN_p2	TTTTGTTTTGGCGACATGGC	CTCCTCCCCCATACTACGAG	45.0	60.0	58.4	58.1	848-884 bp	1.0	85.0
trnC-GCA-petN_p3	TTTTGTTTTGGCGACATGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.4	58.2	795-831 bp	1.0	85.0
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	CTCCTCCCCCATACTACGAG	50.0	60.0	57.5	58.1	815-851 bp	1.0	84.6
trnC-GCA-petN_p5	GACATGGCCAAGTGGTAAGG	CTCCTCCCCCATACTACGAG	55.0	60.0	58.5	58.1	836-872 bp	1.0	84.2