Hotspot

atpH-atpI

LSC · 13534-14266 · 733 bp

Back to Hydrocharis

Pi 0.0627

MarkerSeek score 85.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 65
pi: 0.0627
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	820-1034 bp	1.0	87.0
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	821-1035 bp	1.0	85.8
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.3	57.9	830-1044 bp	1.0	85.1
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	ATCCATGGAGGGTCATCACT	45.0	50.0	58.0	57.8	787 bp	0.333333	51.4
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	ATCCATGGAGGGTCATCACT	45.0	50.0	58.2	57.8	788 bp	0.333333	50.2