Hotspot

petA

LSC · 64369-65331 · 963 bp

Back to Hydriastele

Pi 0.00312

MarkerSeek score 60.50

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 3
pi: 0.00312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	TCCGGGATCAATGATTGGAC	TCGATCGACAATAATTTGTTGCT	50.0	34.8	57.4	57.2	1039 bp	1.0	44.8
petA_p2	TCCGGGATCAATGATTGGAC	TGTTGCTTTTTACCAACTTGATGT	50.0	33.3	57.4	58.0	1023 bp	1.0	42.9
petA_p3	TCCGGGATCAATGATTGGAC	TCGATCGACAATAATTTGTTGC	50.0	36.4	57.4	55.8	1039 bp	1.0	41.7
petA_p4	TCCGGGATCAATGATTGGAC	TCGATCGACAATAATTTGTTGCTT	50.0	33.3	57.4	57.8	1039 bp	1.0	41.7
petA_p5	TCCGGGATCAATGATTGGAC	CGATCGACAATAATTTGTTGCT	50.0	36.4	57.4	55.8	1038 bp	1.0	41.6